STEPS TO SOLVING A CRYSTAL STRUCTURE

  1. Prepare milligram (soon this requirement will be less) quantites of pure ("95%" or greater) protein or protein complex
    1. protein must be in a buffer in which it is stable and has biological activity, if that assay is possible
    2. protein must be a single species (monomer, dimer, heterodimer, whatever it's state is) on a gel filtration/sizing column or dynamic light scattering
  2. Bring, or send protein to our facility
  3. Robotic crystallization screens will be done to find initial crystallization conditions Gilson C-240 96-well crystallization robot
  4. You will eventually be able to visualize your crystallization experiments remotely as they develop
  5. We will help you optimize your crystallization conditions to produce single crystals and find solvents for cryogenic data collection
  6. We will analyze your crystals on our home lab source to determine their diffraction quality
  7. We will help you collect a native data set or help you gain access to the SER-CAT beamlina at APS
  8. We will teach you to process data and carry our molecular replacement with an appropriate model, or else help you prepare heavy atom derivatives