American Cancer Society BE-54B "Structural Enzymology of Pyrimidine Metabolism". C.W. Carter, Jr. P.I. 15% effort. Direct costs for the current year: $86,400. Total direct costs: $172,800 for the period January 1994-31 December 1995.
This project entails X-ray crystallographic studies of Cytidine Deaminases and Orotidylate Decarboxylase complexed to a series of inhibitors related to their catalytic cycles.
National Science Foundation MCB-9304674 "Bayesian Methods for Macromolecular Phase Determination". C. W. Carter, Jr., P.I. 15% effort Direct Costs for the current year: $92,000. Total direct costs: $320,000 for the period August 1993 - July 1996.
This project concerns development and eventual distribution among the protein crystallography community of the MICE program written by Chris Gilmore and implementing the phasing concepts developed by Gerard Bricogne, and which we have exploited in both the Cytidine Deaminase and Tryptophanyl-tRNA synthetase structure determinations.
National Institutes of Health RO1-GM48519 "Trp-tRNA Ligase: X-ray Studies of the Catalytic Cycle. C. W. Carter, Jr., P.I. 10% effort Direct costs for the current year: $116,853. Total direct costs: $499,900 for the period September 1994 - August 1998
This grant supports work on the B. stearothermophilus Tryptophanyl-tRNA synthetase and its complexes with substrates.
NASA GP29H "Quantitative Statistical Methods for Pre-Flight Optimization, and Post-Flight Evaluation of Macromolecular Crystal Growth". C. W. Carter, Jr., P.I. 12% effort. Direct costs for the first year: $198,172. Total direct costs: $569,023.
This proposal deals with specialized techniques for optimizing experimental procedures using response-surface methods and statistical analysis using linear models. It involves development and application of experimental designs and statistical analytical methods similar to those described in Carter and Yin, 1994, Acta Cryst. D50:572-590. The funds also support a series of workshops where participants, especially those planning to fly crystal growth experiments on the shuttle, can learn to use these methods to improve reproducibility of their flight experiments.